Simultaneous Determination of Para Red and Four Sudan Dyes Residues in Eggs by Dispersive Solid-phase Extraction Combined with High Performance Liquid Chromatography
  
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DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:dispersive solid phase extraction(dSPE)  para red  sudan dyes  egg  high performance liquid chromatography(HPLC)
  
AuthorInstitution
朱浩,李小平,邹宝波,陈晓红,陈泰宇,金米聪 1.宁波大学医学院;2.宁波市疾病预防控制中心宁波市毒物研究与控制重点实验室;3.Westtown School
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Abstract:
      A method was established for the simultaneous determination of para red,sudan Ⅰ,sudanⅡ,sudan Ⅲ,and sudan Ⅳ residues in eggs by dispersive solid phase extraction(dSPE) combined with high performance liquid chromatography(HPLC).The samples were extracted with n-hexane by ultrasonic technique,and then enriched and cleaned up with Diol silica gel absorbent.The separation of the target compounds were performed on a Phenomenex Luna C18 column(50 mm×2.0 mm,5 μm) by using acetonitrile-water as mobile phase by gradient elution.The detection of the target compounds was performed with an ultraviolet-visible(UV-vis) detector at 500 nm and quantified by the external standard method.The results showed that the linear ranges of para red and four sudan dyes were between 0.1 mg?L-1and 10.0 mg?L-1 with correlation coefficients more than 0.999.The average recoveries at three spiked levels ranged from 81.2% to 94.2% with relative standard deviations(RSDs) of 3.4%-5.3%.The limits of detection(LODs) were in the range of 0.018-0.030 mg?kg-1,and the limits of quantitation(LOQs) were 0.06-0.10 mg?kg-1.The developed method is simple,rapid and sensitive,and is suitable for the determination of trace dyes residues in eggs.
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