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| Simultaneous Determination of Monomethyl Fumarate and Dimethyl Fumarate in Chinese Herbal Medicine by High Performance Liquid Chromatography |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:high performance liquid chromatography(HPLC) Chinese herbal medicine monomethyl fumarate dimethyl fumarate residual detection |
| Author | Institution |
| 唐柏彬，郗存显，邹芸，陈冬东，王国民，李贤良，张雷，张进忠* |
1.西南大学资源环境学院三峡库区生态环境教育部重点实验室;2.重庆出入境检验检疫局 重庆市进出口食品安全工程技术研究中心;3.重庆市市政环卫监测中心;4.中国检验检疫科学研究院 |
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| Abstract: |
| A high performance liquid chromatographic(HPLC)method was developed for the simultaneous determination of monomethyl fumarate(MMF)and dimethyl fumarate(DMF)in Chinese herbal medicine.Samples were extracted with ethyl acetate，purified with NH2-carb solid phase extraction column，separated with C18 column，and determined with diode array detector(DAD).The results showed that there were good linear relationships between peak areas and concentrations of MMF and DMF in the range of 0.025-5.0 μg/mL，with limits of detection(LODs)of 0.015 and 0.020 mg/kg，and limits of quantitation(LOQs)of 0.05 mg/kg and 0.06 mg/kg，respectively.The mean recoveries of MMF and DMF at spiked levels of 0.1，0.2，0.5 mg/kg were in the range of 78.9%-97.3%，with relative standard deviations(RSDs，n=6)of 1.7%-6.0%.This method is sensitive and reliable，and could meet the requirements for simultaneous detections of MMF and DMF in Chinese herbal medicine. |
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