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| Rapid Identification of Phospholipids in Rat Plasma by UPLC/Q-TOF MS |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:UPLC/Q-TOF MS plasma phospholipids |
| Author | Institution |
| WANG Qiu,FU Lei,ZHANG Yu,TIAN Qing-long,RUI Wen,FENG Yi-fan* |
1.广东药学院中心实验室;2.郑州大学药学院 |
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| Abstract: |
| An ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometric method(UPLC/Q-TOF MS） was developed for rapid separation and identification of phospholipids in rat plasma.The classical Bligh-Dyer was used as extraction method.The target compounds were separated on a BEH C18 column by gradient elution using 10 mmol/L ammonium formater(A）-10 mmol/L ammonium formate acetonitrile/isopropanol(1∶1） (B） as mobile phase,and detected by MS under ESI+ and ESI- mode. 81 compounds were identified by UPLC/Q-TOF MS based on MS/MS fragment ions information,elemental composition in Masslynx 4.1 and the Lipid Maps database,among which 7 PE,7 PI and 1 SM were obtained,and the rest were all PC.The method was rapid，simple and credible for the identification of phospholipids in rat plasma,and could provide a foundation for the research of phospholipids metabonomics. |
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