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| Fluorescence Quenching Reaction of Cefotaxime Sodium with Acriflavine and Their Analytical Applications |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:cefotaxime sodium acriflavine fluorescence quenching |
| Author | Institution |
| FU Sheng-hui,LIU Zhong-fang,LIU Shao-pu* |
1.重庆市巴南区环境监测站;2.西南大学化学化工学院 |
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| Abstract: |
| In Britton-Robinson（BR） buffer solution at pH 2.1-4.1,acriflavine（AF） reacted with cefotaxime sodium（CFTM） to form an ion association complex that quenched the fluorescence of acriflavine and whose compound ratio was 1∶1.The measurement wavelength of fluorescence quenching was located at λex/λem=266 nm/506 nm.The linear range for CFTM ranged from 0.07 mg/L to 5.0 mg/L,and its detection limit was 0.021 mg/L.In this study,the fluorescence spectra characteristics,the optimum reaction conditions and effect of foreign substances were investigated.The composition of ion-association complexes was discussed.Based on the ion-association reaction,a high sensitive,simple and rapid method for the determination of CFTM was proposed.The method was applied for the determination of CFTM in human serum and urine samples with satisfactory results. |
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