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| High Performance Liquid Chromatographic Separation of Zopiclone Enantiomers Using Chiral Mobile Phase Additive |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:high performance liquid chromatography chiral mobile phase additive zopiclone β cyclodextrin chiral separation |
| Author | Institution |
| YE Xiang-xi, SONG Yan-xi*, CHEN Xu-tao, JI You-zhi, SUN Lu-lu, WU Xiao-qian, DU Chun-yan |
东华大学环境科学与工程学院 |
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| Abstract: |
| The chiral separation of zopiclone was studied by using reversed-phase high performance liquid chromatography with β-cyclodextrin（β-CD） as chiral mobile additive.Effects of some factors such as concentration of β-CD,content of methanol,pH value,flow rate and temperature on resolution were investigated.The experimental conditions were investigated by the single variable method.The best chromatographic conditions were as follows:column:Inertsil ODS-SP（5 μm,4.6 mm×250 mm）,organic phase:methanol,mobile phase:20 mmol/L phosphate buffer containing 15 mmol/L β-CD-methanol（85∶15）,flow rate:0.8 mL/min,pH value:5.5,temperature:35 ℃,detection wavelength:305 nm,injection volume:20 μL.Under the optimal conditions,the baseline separation of zopiclone enantiomers was obtained with retention time of 67.42 min and 74.48 min,and resolution of 1.62.The method is simple,sensitive and economic,and could provide a new approach for the further study of zopiclone enantiomers. |
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