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| Determination of Organic Acids in Tobacco by Solid Phase Extraction and Gas Chromatogarphy |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:solid phase extraction enrichment gas chromatography（GC） tobacco organic acids |
| Author | Institution |
| ZHANG Xia,LIU Zhi-hua,YANG Guang-yu,YANG Liu,DUAN Yuan-xing,LIU Chun-bo,CHEN Yong-kuan,MIAO Ming-ming* |
云南烟草科学研究院云南省烟草化学重点实验室 |
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| Abstract: |
| A new method for the determination of 9 organic acids in tobacco was developed by derivatization,solid phase extraction and gas chromatography（GC）.The tobacco samples were methylated with methanol and sulfuric acid.The organic acid ester was firstly extracted using solid-phase extraction in strong acidic medium,and then enriched with MCI-GEL and eluted with methanol.The eluent was determined by GC with a DB 5 capillary column and a splitless injection volume of 1.0 μL at a detector temperature of 250 ℃.This method was applied in the determination of lactic acid,oxalic acid,propandioic acid,acetylpropionic acid,malic acid,citric acid,palmic acid,linoleic acid and stearic acid in tobacco samples.The recoveries were in the range of 92.2%-102.6% with relative standard deviations（RSD） of 2.4%-3.2%.The limits of quantitation（LOQ） for all analytes were between 0.20 mg/L and 0.36 mg/L.The method was accurate and sensitive,and could meet the actual requirements for the analysis of tobacco samples. |
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