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| Study on Determination of Oxalate in Urine by Ion Chromatography in the Presence of Ascorbic Acid |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:ion chromatography oxalate ascorbic acid urine |
| Author | Institution |
| HANG Yi-ping,LU Zhu-liang-zi,YANG Chun-ying |
华南理工大学化学与化工学院 |
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| Abstract: |
| Urinary oxalate was successfully determined by ion chromatography with suppressed conductivity detector in the presence of ascorbic acid.The sample was diluted with deionized water,and filtrated through a 0.22 μm filter.Then the sample was directly separated with IonPac AS12A anion exchange column（200 mm×4 mm） employing 15 mmol?L-1 NaHCO3（pH 8.3） as eluent,and detected with conductivity detector.The result showed that oxalate could be well separated from other anions in urine within 16 min.Under the same condition,urinary ascorbic acid does not decompose into oxalate within 80 min,thus having no influence on the determination of oxalate.The calibration curve was linear in the range of 0.2-40 mg?L-1 with a correlation coefficient（r） of 0.999 9.The detection limit was 0.02 mg?L-1.The average recoveries were in the range of 96.9%-103.3%.The method was rapid,selective and sensitive,and was applied in the determination of urine samples with satisfactory results. |
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