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| Determination of Bisphenol A-type Compounds in Canned Foods by High Performance Liquid Chromatography with Fluorescence Detection |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:food bisphenol A-type compounds high performance liquid chromatography(HPLC) fluorescence detection |
| Author | Institution |
| FANG Xiao-ming*,ZHOU Hao,FENG Chun-ye,TANG Yi-feng,DING Zhuo-ping |
1.上海出入境检验检疫局;2.欧普图斯光学纳米科技有限公司;3.上海海洋大学 食品学院 |
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| Abstract: |
| A method for the determination of 7 bisphenol A-type compounds in canned foods by high performance liquid chromatography with fluorescence detection（HPLC/FLD） was established.Samples were extracted with acetonitrile,and cleaned up with a polyvinylpyrrolidone（PLS） cartridge.Chromatographic separation was carried out on a phenyl column（250 mm×4.6 mm,5 μm） with acetonitrile-water as mobile phase by gradient elution.The baseline separation of seven bisphenol A-type compounds was obtained.Excitation and emission wavelengths of fluorescence detection were set at 227 nm and 313 nm,respectively.For seven bisphenol A-type compounds,there were good linear relationships between concentrations and peak areas in the range of 0.010-5.0 μg/mL,and the correlation coefficients（r2） were greater than 0.99.The average recoveries of 7 bisphenol A-type compounds at fortified levels of 0.015-5.0 mg/kg ranged from 83.7% to 95.5%,with relative standard deviations（RSDs） of 7.2%-19.8%.The limits of detection（LOD） and quantitation（LOQ） were in the range of 5-18 μg/kg and 15-50 μg/kg,respectively.This method was accurate,sensitive and reliable,and could be applied in the real sample analysis. |
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