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| Electrogenerated Chemiluminescence Determination of Dopamine Using Screen printed Carbon Electrodes |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:electrogenerated chemiluminescence (ECL) screen printed carbon electrode dopamine borate buffer |
| Author | Institution |
| ZHENG Rui-juan*,ZHONG Jian-hai,QIU Ze-hai,LING Bao-long,ZHANG Wen-bin |
1.龙岩学院化学与材料学院; 2.国家矿产品检测重点实验室 |
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| Abstract: |
| Screen printed carbon electrode(SPCE) herein was fabricated by screen printing technique,and then activatedly pretreated by soaking it into concentrated NaOH solution.The treatment effect was evaluated by electrochemical impedance method and cyclic voltammetry.The result showed that the electron transfer resistance(Rct) decreased with the soaking time.The peak to peak separation(Ep) in cyclic voltammetry was reduced from 400 mV to 84 mV,and the peak current was gradually increased.The ratio between oxidation peak current and reduction peak current was close to 1∶1,indicating that the electrochemical reversibility was greatly promoted.In addition,compared with that of the fresh SPCE unactivated,electrogenerated chemiluminescence(ECL) of ［Ru(bpy)3］2+ was enhanced by a factor of 36 after pretreatment in concentrated NaOH solution.The experimental results also showed that the ECL of ［Ru(bpy)3］2+ was promoted after adding dopamine into borate buffer without coreactants.Based on this principle,a novel ECL sensor for determination of dopamine was constructed.Effect of pH value was studied.Under the optimized conditions,the ECL intensity showed a good linear relationship toward dopamine over the concentration range of 1.0×10-10-5.0×10-7 mol/L with a limit of detection of 5.0×10-11 mol/L.This proposed method was applied in the analysis of dopamine in practical injection samples,with recoveries of 95% - 102%.The method was simple and cost effective,and could realize batch production in design and in operation with high sensitivity and stability. |
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