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| Determination of 18 Sulfonamides in Veterinary Pharmaceutical Powder by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:sulfonamides high performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS） veterinary pharmaceutical powder |
| Author | Institution |
| WANG Xiao-li，GUO Tao，WANG Shan-shan，ZHAO Jin，YUAN Jin-peng，ZHAO Ru-song |
1.山东省科学院山东省分析测试中心;2.山东省农业科学院山东省水稻研究所 |
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| Abstract: |
| A high performance liquid chromatography-electrospray ionization triple-quadruple tandem mass spectrometric（HPLC-ESI-MS/MS） method was established for the determination of 18 sulfonamides and their potentiators in veterinary pharmaceutical powder，including sulfadimidine，sulfacetamide，sulfamethizole，sulfachloropyridazine，sulfamethoxazole，sulfathiazole，sulfamonomethoxine，sulfamerazine，sulfadoxine，sulfapyridine，sulfameter，sulfamethoxypyridazine，sulfaphenazolum，sulfadimethoxypyrimidine，sulfisoxazole，sulfaquinoxaline，sulfadiazine and trimethoprim.The 18 banned additives were extracted with methanol-H2O（90∶10）.Then the reconstituted sample solution was analyzed by HPLC-MS/MS under positive ion mode，with a C18 column（2.1 mm×150 mm，5 μm） as analytical column.The detection limits of 18 sulfonamides were all 10 μg/kg.The calibration curves were linear in the range of 50-2 000 μg/kg，with correlation coefficients more than 0.99.The method was validated at spiked levels of 50，200,1 000 μg/kg，and the overall recoveries were in the range of 86.8%-115% with relative standard deviations（RSDs） of 1.0%-9.7%. |
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