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| Synthesis of 2-（2-Hydroxy-3-methoxyl phenyl）-5-（1-hydroxymethyl） C60 Pyrrolidine Derivative and Its Application in Electrochemical DNA Sensors |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:C60 pyrrolidine derivative DNA sensor aluminum ion methylene blue |
| Author | Institution |
| YUAN Xiao-ning,QIU Wei-wei,LI Fu-xiao,XIE Li-qing,XU Piao-yang,GAO Feng,WANG Qing-xiang,GAO Fei |
闽南师范大学化学与环境学院 福建省现代分离分析科学与技术重点实验室 |
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| Abstract: |
| A new compound 2-（2-hydroxy-3-methoxyl-phenyl）-5-（1-hydroxymethyl） C60 pyrrolidine derivative（FPD） was successfully synthesized with 1,3-dipolar cycloaddition reaction of C60 with o-vanillin and L-serine.Its structure was characterized by MS,FT-IR,UV-Vis,1H NMR and elemental analysis.FPD was immobilized on the surface of glassy carbon electrode（GCE） by drop coating method,and then Al3+was assembled on its surface.The probe DNA was fixed on the electrode surface by the electrostatic interaction between the negatively charged phosphate group of DNA and Al3+,and a new electrochemical DNA sensor was constructed successfully.The analytical performance of the biosensor was evaluated using methylene blue as the hybridization indicator.The results showed that a good linear relationship between peak currents signals of MB and logarithm value of the target compound concentrations（lgcS2） in the range of 1.0×10-15-1.0×10-9 mol/L was obtained,with a linear regression equation of Ip（10-6 A）=0.106 lg（cS2，mol/L）+0.735 and a limit of detection（S/N=3） of 5.4×10-16 mol/L.The selectivity experiments further showed that the complementary sequences and the base mismatch sequences could be well distinguished with this biosensor. |
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