Simultaneous Determination of Nucleosides and Nucleobases in Polygoni Multiflori Radix from Different Origins by UPLC-QTRAP-MS/MS
  
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DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:Polygoni Multiflori Radix  UPLC-QTRAP-MS/MS  origins  nucleosides  nucleobases  simultaneous determination
  
AuthorInstitution
LUO Yi-yuan,LIU Juan-xiu,LIU Xun-hong,LAN Cai-wu,HOU Ya,MA Yang,XU Li 1.南京中医药大学药学院;2.贵州昌昊中药发展有限公司研发部;3.扬州市药品检验所化学室
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Abstract:
      A comprehensive analytical method based on ultra performance liquid chromatography-quadrupole linear ion trap mass spectrometry(UPLC-QTRAP-MS/MS) was developed for the simultaneous determination of 10 nucleosides and nucleobases(uracil,cytidine,guanine,uridine,adenine,inosine,guanosine,thymidine,adenosine,2’deoxycytidine)in Polygoni Multiflori Radix.The sample was extracted with ultrapure water by ultrasonic machine at room temperature and centrifuged.Under the optimized conditions,good separations for 10 target compounds were obtained on a Waters Atlantis T3 column(2.1 mm×150 mm,3 μm)with a mobile phase of methanol-5 mmol/L ammonium acetate(0.1% acetic acid) at a flow rate of 0.4 mL/min.The target compounds were analyzed in the positive ion multiple reaction monitoring(MRM) mode.As a result,10 nucleosides and nucleobases obtained good linearities in the certain concentration ranges with correlation coefficients more than 0.99,and the limits of detection were in the range of 1.05-9.68 ng/mL.Average recoveries of 10 compounds were between 97.8% and 104.8%,and the relative standard deviations were between 1.8% and 5.0%.The method was simple,sensitive and accurate,and could provide a reliable and effective technique for the quality control of Polygoni Multiflori Radix.
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