|
| Simultaneous Determination of Five Dyes in Chilli Powder by Ultrasonic-assisted Dispersive Emulsion Microextraction Coupled with High Performance Liquid Chromatography |
| |
| View Full Text Download reader |
| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:Para red Sudan red ultrasonic-assisted dispersive emulsion microextraction high performance liquid chromatography(HPLC) |
| Author | Institution |
| SU Ri-hui,RUAN Gui-hua,ZENG Ling-zhen,CHEN Zheng-yi,DU Fu-you |
桂林理工大学化学与生物工程学院 |
|
| Hits: 2265 |
| Download times: 436 |
| Abstract: |
| A method based on ultrasonic-assisted dispersive emulsion microextraction procedure coupled with high performance liquid chromatography(HPLC) was developed for the preconcentration and determination of Para red,Sudan Ⅰ,Sudan Ⅱ,Sudan Ⅲ and Sudan Ⅳ in chilli powder.Experimental conditions affecting the microextraction procedure such as types and volumes of extraction solvent,content of disperser solvent,salt concentration,pH value,extraction time and centrifugal time were optimized.The ultrasonic-assisted dispersive emulsion microextraction conditions were optimized by using 0.75 mL cyclohexane as extraction solvent and 10% acetonitrile as disperser solvent,and the mixture was oscillated for 1 min.The extractant phase was separated by centrifugation at 6 000 r/min for 5 min,then filtered through 0.22 μm nylon membranes and injected into the HPLC system for analysis.Under the optimum conditions,this method was employed to analyze five dyes in loose chilli powder and packaging chilli powder.At three spiked levels of 50-1 000 ng/g,the average recoveries for five dyes in chilli powder ranged from 82% to 114%,with relative standard deviations(RSD,n=3) between 0.7% and 8.8%.The calibration curves of 5 dyes were linear in the concentration range of 25-5 000 ng/mL with correlation coefficients(r2) not less than 0.998 9 and limits of detection(LOD) of 1-11 ng/mL.This method is convenient,sensitive and accurate,and is suitable for the determination and confirmation of 5 dyes in chilli powder． |
| Close |
|
|
|