Multi-residue Determination of 295 Pesticides in Tea and Its Products by Gas Chromatography-Mass Spectrometry and Gas Chromatography
  
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KeyWord:gas chromatography(GC)  gas chromatography-mass spectrometry(GC-MS)  sample pretreatment  multiresidual analysis  tea  tea powder
  
AuthorInstitution
SU Jian-feng*,ZHONG Mao-sheng,CHEN Jing,GUO Xin,CHEN Jin-xing,LIANG Zhen,LIU Jian-jun 1.福建中检华日食品安全检测有限公司;2.宁德出入境检验检疫局;3.福建出入境检验检疫局;4.中国检验认证集团福建有限公司
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Abstract:
      A method was developed and applied to determine the residual amounts of 286 pesticides(organophosphorus,organochlorine,pyrethroid,carbamate and other pesticides) in tea and its products by gas chromatography-mass spectrometry(method Ⅰ),and an other method was applied to determine 9 kinds of strongly polar organophosphorus pesticides in tea and its products by gas chromatography(method Ⅱ).For method Ⅰ,the residues were extracted from homogenized tissue with ethyl acetate assisted by ultrasonication.The clear supernatant liquid purified by solvent transfer technique and solid phase extraction(Cleanert TPT column) was ready for GC-MS analysis.For method Ⅱ,the residues were extracted from homogenized tissue using acetonitrile assisted by ultrasonication.The supernatant was purified by solid phase extraction(Cleanert TPT column) prior to GC analysis.Determinations were performed by GC-MS in selected ion monitoring(SIM) mode and GC method with flame photometric detector(FPD),then external standard method was used in the quantification.The methods were convenient and rapid after the conditions of pretreatment and determination were investigated.Under the optimal conditions,the quantitation limits of two methods(S/N≥10) for pesticides were in the range of 0.01-0.10 mg/kg.The linear ranges were between 0.01 mg/L and 1.00 mg/L with correlation coefficients(r2) larger than 0.99.Tests for recovery were carried out by addition of standards at concentration of 0.10 mg/kg,the recoveries of method I were in the range of 51%-135%,among which the recoveries for 88.3% pesticides were between 70% and 110%,and the recoveries of method Ⅱ were in the range of 68%-99%,while the relative standard deviations were in the ranges of 3.7%-18% and 3.5%-8.2%,respectively.The methods are easy,fast and sensitive,and could meet the requirements of the multiresidual analysis in tea and its products.
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