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| Determination of Taraxerol in Mangrove Sediments by GC-MS with Ultrasonic Extraction |
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| DOI： |
| KeyWord:gas chromatography-mass spectrometry(GC-MS) ultrasonic extraction mangrove sediments taraxerol |
| Author | Institution |
| ZHANG Dao-lai,WANG Xu-ling,JIANG Xue-jun,HE Xing-liang,MENG Xian-wei,WANG Xiao-ru,LIN Xue-hui* |
1.国土资源部海洋地质油气资源与环境地质重点实验室;2.青岛海洋地质研究所;3.青岛大学化学化工与环境学院;4.国家海洋局第一海洋研究所 |
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| Abstract: |
| A gas chromatography-mass spectrometric method with ultrasonic extraction was developed for the determination of taraxerol in mangrove sediments.The parameters including instrument condition,extraction,saponification,derivatization and data analysis were optimized.The analyte was separated by GC with an HB-5MS capillary column,then identified and quantified by the characteristic chromatograms properties and retention time.The component was separated completely from others,simultaneously identified and quantified with the standard curves.Quantitation using internal standard was based on the ratio of peak area of taraxerol derivative to peak area of internal standard derivative in the selected ion monitoring mode with electron impact source.The calibration curve was linear in the range of 5-100 μg/L,with a limit of quantitation of 0.5 μg/kg.The mean recoveries at 5,20,80 μg/L spiked levels were in the range of 81.2%-108.3%,with relative standard deviations of 1.9%-4.2%.The results showed that the developed method was sensitive,accurate and reproducible,and was suitable for the analysis of taraxerol in mangrove sediments.The study could provide scientific evidences for the management and protection of mangrove. |
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