Determination of Benzo[α]pyrene in Sarcandra Galbra by Solid Phase Extraction/High Performance Liquid Chromatography with Fluorescence Detector
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KeyWord:benzo[α] pyrene  Sarcandra galbra  solid phase extraction  high performance liquid chromatography/fluorimetric detection
LIN Pu,LIANG Yi-chi,HU Juan* 福建中医药大学药学院
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      A method was developed for the determination of benzo[α] pyrene in Sarcandra galbra by solid phase extraction/high performance liquid chromatography(HPLC) with fluorescence detector.The benzo[α] pyrene in Sarcandra galbra was extracted with n-hexane,and purified with neutral alumina column.The prepared samples were analyzed by HPLC with fluorescence detector at λex=370 nm and λem=425 nm,using acetonitrile-water(85∶15) as mobile phase at a flow rate of 1.0 mL/min.The results showed that a good linearity was achieved in the range of 0-20 ng/mL for benzo[α] pyrene with a correlation coefficient of 0.999 9.The limit of quantitation(LOQ) was 0.36 μg/kg.The mean recoveries of benzo[α] pyrene were in the range of 84.4%-101.9% with relative standard deviations(RSDs) of 1.6%-5.6%.The method is accurate,sensitive and repeatable,and is suitable for the analysis of benzo[α] pyrene in traditional Chinese herbal medicines.