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| Amplified Optical Aptasensor through Exonuclease Ⅲ Stimulated Regeneration of ATP Based on Graphene |
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| DOI： |
| KeyWord:adenosine triphosphate exonuclease Ⅲ graphene fluorometry aptasensor |
| Author | Institution |
| WEN Chang-chun*,ZHAO Shu-lin,HUANG Yong,PAN Li,CHEN Jia,HU Kun |
广西师范大学化学与药学学院药用资源化学与药物分子工程教育部重点实验室 |
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| Abstract: |
| A highly sensitive platform for adenosine triphosphate(ATP)assay was designed based on exonuclease Ⅲ(Exo Ⅲ)induced target recycling amplification and fluorescence quenching of graphene.The system mainly consists of two nucleic acid strands P1 and P2 carrying a part of aptamer sequences for ATP,and P1 with FITC labeled on its 3’ end.Upon addition of target ATP to the system,P1 hybridizes with P2,and forms a DNA duplex sandwich structure.The formation of DNA duplex sandwich structure triggers selective enzymatic cleavage of 3-recessed end by Exo Ⅲ,resulting in the release of target ATP and FITC.Released target ATP then hybridizes with nucleic acid strands P1 and P2,generating many FITC molecule.As a result,the FITC molecule break away from the G surface,and lead to the fluorescence recovery of the dye FITC.This assay exhibits a high sensitivity with a detection range of 0.02-1 μmol/L and a detection limit(S/N=3) of 9 nmol/L. |
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