Characterization of Flavone di-C-glycosides in Viola yedoensis by Ultrahigh Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight-Mass Spectrometry
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KeyWord:Viola yedoensis  flavone C-glycoside  quadrupole time of flight-mass spectrometry(QTOF-MS)  liquid chromatography-mass spectrometry(LC-MS)
QIN Yan,YIN Jian-bing,DU Dong-sheng,CAO Jie,YIN Cheng-le,CHENG Zhi-hong* 1.复旦大学药学院生药学教研室;2.浙江省医疗器械不良事件监测中心
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      MS/MS fragmentation patterns of five flavone di-C-glycoside standards from Viola yedoensis were studied by ultrahigh performance liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry(UPLC-ESI-QTOF-MS/MS) in the negative ion mode.The fragmentation pathways of C-6 and C-8 sugars in these flavone di-C-glycosides were consistent with those reported earlier.The results of our study also suggested that the configurations of sugars at C-8 obviously influenced the fragmentation behaviors.The fragmentation behaviors of these flavone di-C-glycoside standards were successfully used to characterize flavone C-glycosides in V.yedoensis.Total of 21 flavone di-C-glycosides along with 4 flavonol O-glycosides were identified or tentatively identified on the base of their MS/MS data,accurate molecular weights,or by comparing with reference standards.The UPLC-ESI-QTOF-MS/MS method is rapid,sensitive and accurate,and could be used for the characterization of flavone C-glycosides in traditional Chinese medicines.