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| Determination of Eight Mycotoxins in Rice by Dispersive Solid Phase Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:QuEChERS method EMR-lipid method DisQuE method liquid chromatography-tandem mass spectrometry(LC-MS/MS) mycotoxins rice |
| Author | Institution |
| SUN Wei-hua,YANG Huan,CAO Zhao-yun,MA You-ning,QIN Mei-ling,CHAI Shuang shuang,CHEN Ming-xue* |
中国水稻研究所农业部稻米及制品质量监督检验测试中心 |
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| Abstract: |
| A rapid method was developed for the determination of HT-2 toxin,T-2 toxin,fumonisin B1,fumonisin B2,zearalenone,ochratoxin A,deoxynivalenol and aflatoxins B1 in rice.Three dispersive solid phase extraction sample preparation methods,including QuEChERS method,EMR-lipid method and DisQuE method,were compared for the determination of 8 mycotoxins in rice.The recoveries of the three extraction methods for the toxins were studied.The method of after-extraction addition was used to evaluate the matrix effects for mycotoxins in rice by liquid chromatography-tandem mass spectrometry(LC-MS/MS).As a result,the QuEChERS method was not suitable for the analysis of fumonisin B1,fumonisin B2 and ochratoxin A.The EMR-lipid methods could not overcome the matrix effects of zearalenone and ochratoxin A in rice.In this study,mycotoxins were extracted by DisQuE method,and analysed by LC-MS/MS under the optimized condition with monitoring 16 pairs of MS/MS transitions of precursor ions(two for each mycotoxin) in one single run.Recoveries at three fortified levels in rice ranged from 70.0% to 124.1% with relative standard deviations of 0.9%-16.9%.The limits of detection(S/N≥3) were in the range of 1.2-60.0 μg/kg.The method was proved to be highly efficient,robust and sensitive,and was suitable for the simultaneous analysis of mycotoxins in rice. |
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