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| Studies on Interaction of Tyr-Tyr-Tyr-Tyr with ct-DNA |
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| DOI： |
| KeyWord:Tyr-Tyr-Tyr-Tyr ct-DNA interaction Fmoc solid-phase methods |
| Author | Institution |
| SUN Zhi-jie,L? Ming-xiu ,LU Kui*，DUAN Bing-chao,LIU Guang-bin |
1.郑州大学化学与分子工程学院;2.河南工程学院材料与化学工程学院 |
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| Abstract: |
| A model peptide,Tyr-Tyr-Tyr-Tyr（YYYY）,was synthesized by Fmoc solid-phase methods,purified and separated by reverse phase high performance liquid chromatography（RP-HPLC）,and characterized by ESI-MS analysis.The interaction of peptide with calf thymus DNA(ct-DNA) was investigated by UV-Vis absorption spectra,circular dichroism(CD) and fluorescence spectra.The results showed that,with the increase of peptide concentration,there were a hyperchromic effect on UV spectra,a remarkable fluorescence quenching and a red shift of CD.It is possible that Tyr-Tyr-Tyr-Tyr and Tyr might interact with ct-DNA via the groove binding mode.The interaction between Tyr-Tyr-Tyr-Tyr and ct-DNA,compared with that between Tyr and ct-DNA,is more stronger. |
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