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| Application of Hydrophilic Interaction Chromatography in Analysis of Carbohydrates |
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| DOI： |
| KeyWord:hydrophilic interaction chromatography stationary phase retention mechanism carbohydrate analysis review |
| Author | Institution |
| PU Jiang-hua,ZHAO Xia*,HAN Wen-wei,BAI Ming-yue |
中国海洋大学海洋药物教育部重点实验室，山东省糖科学与糖工程重点实验室 |
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| Abstract: |
| The analysis of carbohydrate structure and composition plays a vital role in the study of structure-activity relationship of carbohydrates.Hydrophilic interaction chromatography(HILIC) could be used to separate polar compounds like carbohydrates with satisfactory resolution.The stationary phase and retention mechanism of HILIC column commonly used in carbohydrates analysis were introduced.The effects of strong elution solvent ratio,mobile phase pH value,buffer salt concentration and column temperature on separation were also discussed.Application examples in analysis of monosaccharide composition,disaccharide composition of glycosaminoglycans,polymerization degree of oligosaccharides,glycosides,glycolipids,sugar alcohols and N-/O-glycans by HILIC were illustrated in this paper. |
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