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| Preparation of a Molecularly Imprinted Film Modified Electrochemical Sensor for L-threonine Determination |
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| DOI： |
| KeyWord:L-threonine(L-Thr) molecular imprinted polymer nano-silver |
| Author | Institution |
| YUAN Ai-guo,LI Bin,CHEN Jian-ping,XIAO Li-jie，JIN Guan-ping |
1．合肥工业大学化学与化工学院;2．汕头市佳禾生物科技有限公司 |
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| Abstract: |
| A nano-silver/poly quercetin composites modified paraffin-impregnated graphite electrode was prepared by electrochemical method(Ag/Qu/WGE).A mixture of L-Thr,chitosan and Nafion was coated on the surface of Ag/Qu/WGE,L Thr(template molecule) was removed by electrochemical cleaning using potentiostatic method.As a result,a molecular imprinted polymer modified electrode based on chitosan/nano-silver/poly quercetin composites could be obtained for L-Thr detection(TMIP/WGE).The characteristics of the modified electrode were investigated by means of field emission scanning electron microscope technique,X-ray photoelectron spectroscopy,infrared spectra and electrochemical technique.The result indicated that the TMIP/WGE displayed a good electro-catalysis towards the oxidation of L-Thr,which could be used for sensitive and quick detection of L-Thr.The differences of oxidation peak current of L-Thr(1.45 V) showed a good linearity in the concentration range of 0.1-1.0 μmol/L with a detection limit(3σ) of 0.01 μmol/L.The electrode was successfully applied in the determination of L-Thr in fermentation broth. |
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