Preparation of an Electrochemical Biosensor Based on Sodium Hydroxide Etching Glassy Carbon Electrode and Its Detection on E-coli
  
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KeyWord:E-coli  NaOH etching  electrochemical biosensor
  
AuthorInstitution
XU Shi-chao,WEN Jun-nan,JIANG Nan,LI Jiao,WANG Cai-fu,DONG Kai,ZHANG Yi-yi 1.天津工业大学环境与化学工程学院;2.天津工业大学中空纤维膜材料与膜过程省部共建国家重点实验室
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Abstract:
      A high sensitive and selective electrochemical DNA sensor for E-coli was prepared by using NaOH etched glassy carbon electrode(GCE) as the substrate.Carboxyl was brought to the surface of GCE via NaOH etching,which improved DNA probes surface density.DNA probes was immobilized on the surface of GCE through the peptide bond.The sensitivity and selectivity of the sensor were characterized by cyclic voltammetry(CV) and differential pulse voltammetric(DPV) methods.The sensor shows a high sensitive and selective response to ssDNA of E-coli,and its linear range was 12.5-62.5 nmol/L with a detection limit of 1.20×10-9 mol/L.
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