Simultaneous Determination of Ten Plant Growth Regulator Residues in Fruit and Bean Sprout by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Stable Isotope-labelled Internal Standards
  
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KeyWord:ultra performance liquid chromatography-tandem mass spectrometry  plant growth regulator  isotope-labelled internal standards  fruit  bean sprout
  
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刘柏林,谢继安,赵紫微,王秀莉,单晓梅 安徽省疾病预防控制中心
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Abstract:
      A method for the determination of ten plant growth regulator residues in fruit and bean sprout by ultra performance liquid chromatography-electrospray tandem mass spectrometry(UPLC-MS/MS) was established. The samples were extracted by the stirring and ultrasound using formic acid-acetonitrile(1∶1000), and purified with the solid phase extraction column. The analytes were separated by using methanol-5mmol/L ammonium acetate as mobile phase. The identifications were achieved by the electrospray ionization in positive and negative mode(ESI+/ESI-) using the multiple reaction monitoring(MRM).The quantifications were performed by the matrix matched isotope-labelled internal standards. Under the optimal experimental conditions, the calibration curves of ten analytes showed good linearities in the concentration range of 0.1-50.0μg/L with correlation coefficients(r2) above 0.995. Recoveries were between 52.4% and 119.6% with RSDs less than 13.0%.The limits of detection were 0.2μg/kg. The method had the advantages of simple pretreatment, good purification and low matrix effects, and was suitable for the rapid, high-throughput quantitative analysis of plant growth regulator residues in fruit and bean sprout.
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