Rapid Determination of Polybrominated Diphenyl Ethers in Urine of Occupational Worker Using QuEChERS Coupled to Gas Chromatography-Mass Spectrometry
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KeyWord:polybrominated diphenyl ethers(PBDEs)  urine  gas chromatography-mass spectrometry(GC-MS)  occupational worker
郁东,王德军,朱文文,陈田,施致雄 1.山东省职业卫生与职业病防治研究院检验科;2.山东省疾病预防控制中心;3.首都医科大学公共卫生学院
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      A method for the analysis of polybrominated diphenyl ethers(PBDEs) in urine of occupational worker was developed using QuEChERS(quick,easy,cheap,effective,rugged,safe) pretreatment coupled to gas chromatography-mass spectrometry(GC-MS).Urine sample was extracted with an acetone-hexane mixture by dehydration and salting-out using NaCl and anhydrous MgSO4.The purification was performed with C18 particlet,and GC-MS determination was perfomed under negative chemical ionization(NCI) mode.The calibration curves were linear in the ranges of 10-1 000 pg/μL for BDE-209,and 1-100 pg/μL for the rest of analytes,with correlation coefficients higher than 0.999 .The average recoveries were in the range of 91.7%-110.2% at three spiked levels,with relative standard deviations lower than 10%.The limits of detection(LOD) of PBDEs ranged from 0.3 pg/mL to 36 pg/mL.This method was validated to be simple,sensitive and selective,and was suitable for the exposure accessment of pollutant in occupational workers.