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| Rapid Determination of 18 Growth Promoting Agents in Bovine Liver by UPLC-MS/MS Coupled with PRiME HLB SPE Clean-up |
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| DOI： |
| KeyWord:UPLC-MS/MS PRiME HLB solid-phase extraction growth promoting agents bovine liver |
| Author | Institution |
| WANG Zhi，SHI Zong-wei，XI Cun-xian，CAO Shu-rui，WANG Guo-min，TANG Bo-bin，ZHENG Jia，MU Zhao-de |
1.重庆医科大学药学院;2.重庆出入境检验检疫局重庆市进出口食品安全工程技术研究中心;3.重庆市生物化学与分子药理学重点实验室 |
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| Abstract: |
| A method was developed for the rapid detection of 8 hormones，6 β-receptor agonists and 4 antibiotics growth promoting agents in bovine liver by PRiME HLB solid-phase extraction/ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The chromatographic separation conditions，MS/MS detection parameters and sample pretreatment were optimized，respectively.The sample was firstly added NH4OAc buffer solution(pH 5.2)，and hydrolyzed with a mixed solution of β-glucuronidase/sulfatase，then extracted with methanol(MeOH) and acetonitrile(ACN)-MeOH(90∶10，by volume) step by step，finally purified with PRiME HLB.The effluent was collected and concentrated to dryness，and redissolved with ACN-water(3∶7，by volume) for UPLC-MS/MS detection.The results showed that 18 kinds of compounds had good linear relationships in the range of 1.0-100.0 μg/L with their correlation coefficients higher than 0.990.The detection limits(LODs) ranged from 0.07-0.78 μg/kg，and the quantitation limits(LOQs) ranged from 0.23-2.58 μg/kg.The average recoveries of 18 compounds at three spiked levels(0.5，1.0，5.0 μg/kg) were in the range of 67.5%-102.0%with relative standard deviations(RSDs) less than 15%.The method was simple，rapid and accurate，and was suitable for the simultaneous detection of 18 growth promoting agents in animal tissues. |
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