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| Determination of Aflatoxins in Aquatic Feeds Samples by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Multi component Immunoaffinity Column Purification |
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| DOI： |
| KeyWord:multi-component immunoaffinity column ultra performance liquid chromatography-tandem mass spectrometry feeds aflatoxins |
| Author | Institution |
| LIU Wen-jing，YU Hai-xia,YAN Zhong-yong,ZHANG Xiao-jun，DING Guo-fang |
1.School of Food and Pharmacy,Zhejiang Ocean University;2.Marine Fisheries Research Institute of Zhejiang;3.Ocean Research Center of Zhoushan,Zhejiang University |
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| Abstract: |
| An ultra performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS) method was established for the determination of aflatoxins（AFB1,AFB2,AFG1 and AFG2）in aquatic feeds based on multi-component immunoaffinity column purification.The samples were ultrasonically extracted with 80%acetonitrile aqueous solution,then purified with a multi-component immunoaffinity column,finally analyzed by UPLC-MS/MS with electrospray ionization in positive ion mode（ESI+）under multiple reaction monitoring（MRM）mode and quantified by the external standard method.The separation was performed on an Acquity UPLC BEH C18 column by gradient elution with 0.1%formic acid-acetonitrile as mobile phases.Good linear responses were obtained in the concentration ranges of 2.25-22.5 ng/mL for AFB1 and AFG1,and 0.75-7.5 ng/mL for AFB2 and AFG2,with their correlation coefficients larger than 0.997,respectively.The limits of quantitation（LOQs） were 0.7 μg/kg for AFB1 and AFG1,and 0.2 μg/kg for AFB2 and AFG2,respectively.The average recoveries for AFB1 and AFG1 at a spiked level of 1.5 μg/kg,and AFB2 and AFG2 at a spiked level of 0.5 μg/kg ranged from 78.7%to 85.5%,with intra-day and inter-day relative standard deviations of 6.0%-6.5%and 6.6%-7.0%,respectively.The method was simple,rapid,sensitive and reliable,and was suitable for the determination of aflatoxins residues in aquatic feed samples. |
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