以硼和苯基共掺杂的石墨相氮化碳纳米片为模拟酶的过氧化氢荧光传感器

钟坚海; 陈靖; 黄建立; 陈宜; 张艳燕; 温建荣; 苏雪香; 郭良洽

A Fluorescence H2O2 Sensor Based on Boron and Phenyl doped Graphitic Carbon Nitride(g-C3N4) Nanosheets as Peroxidase Mimic

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KeyWord:graphitic carbon nitride codope peroxidase mimic H2O2 fluorescence sensor

Author	Institution
ZHONG Jian-hai，CHEN Jing,HUANG Jian-li，CHEN Yi,ZHANG Yan-yan,WEN Jian-rong,SU Xue-xiang,GUO Liang-qia	1.Longyan Entry-Exit Inspection and Quarantine Bureau Comprehensive Technology Service Center;2.Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology,Fuzhou University，;3.Institute of Grain and Oil Quality Supervision and Test of Fujian

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Abstract:

A novel “turn on” fluorescence sensor for H2O2 detection was developed based on boron and phenyl doped graphitic carbon nitride nanosheets(BP-C3N4) as peroxidase mimic.The BP-C3N4 with peroxidase-like activity were prepared by thermal polymerization of cyanamide with 3-aminobenzeneboronic acid followed by ultrasonic exfoliation.Similar to horseradish peroxidase，the BP-C3N4 could catalyze the oxidation of amplex ultrared reagent(AUR) by H2O2 to generate the resorufin with strong fluorescence.Parameters for the peroxidase like catalytic reaction，including response time，pH value and temperature were further investigated.Under the optimal conditions，the obtained fluorescence signal showed a good linear response to H2O2 concentration over the range of 0-100 μmol/L，with a correlation coefficient(r2) of 0.992 2 and a limit of detection of 1.1 μmol/L.The fluorescence sensor was applied in the detection of H2O2 residue in real samples，and the results were consistent with those of the existed method.