Rapid Screening and Confirmation of 74 Antibiotics and Other Compounds in Swine Urine by Ultra Performance Liquid Chromatography-Quadrupole-Exactive Orbitrap Mass Spectrometry
  
View Full Text    Download reader
DOI:
KeyWord:ultra performance liquid chromatography-quadrupole-exactive orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap)  swine urine  antibiotics and other compounds  rapid screening
  
AuthorInstitution
ZHANG Jing,GONG Song-song,WU Jian-ping,PAN Juan,WANG Bo,YAN Feng Shanghai Municipal Supervisory Institute of Veterinary Drugs and Feedstaff
Hits: 2149
Download times: 1181
Abstract:
      An ultra performance liquid chromatography-quadrupole-exactive orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap) was developed for the rapid screening and confirmation of 74 antibiotics and other compounds in swine urine.The samples were high speedily centrifuged,then diluted with solvent,and finally separated on an Agilent SB C18 (3.0 mm×100 mm,1.8 μm) column using a binary solvent system composed of 0.1% formic acid aqueous solution and 0.1%formic acid acetonitrile solution by gradient elution.Under the optimal conditions,the analytes were precisely screened and confirmed with the precision mass,retention time and two stage ion mass spectra.There were good linear relationships for 74 analytes in their respective concentration ranges with correlation coefficients(r2) larger than 0.99 and limits of detection(LOD) of 10 ng/mL.The recoveries for 74 compounds in swine urine at LOD spiked level mainly ranged from 55.8%to 120%,with relative standard deviations(RSD) of 1.3%-21%.The method was convenient,rapid,sensitive and accurate,and was quite effective for the screening and confirmation of 74 antibiotics and other compounds in swine urine,which was beneficial to the investigation on drugs situation in husbandry.
Close