Rapid Screening of 32 Dyes in Oxidative Hair Dyes by Ultra-performance Liquid Chromatography-Quadrupole/Electrostatic Field Obitrap High-resolution Mass Spectrometry
  
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KeyWord:ultra-performance liquid chromatography  obitrap high resolution mass spectrometry  hair dyes  dye  rapid screening
  
AuthorInstitution
JU Ling-yan,LI Zhao-jie,SONG Wei-de,XU Cheng-gang,CUI Jia 1.Weihai Customs;2.Rizhao Customs
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Abstract:
      A method was developed for the rapid screening and analysis of 32 banned and restricted components in hair dyes by ultra performance liquid chromatography-quadrupole/electrostatic field obitrap high resolution mass spectrometry.The samples were extracted using a mixed solution of methanol and 2 g/L aqueous ascorbic acid,and purified with n hexane.The separation was carried out on an ZORBAX Eclipse Plus C18 column(3.0 mm×100 mm,1.8 μm)by gradient elution with methanol and 5 mmol/L ammonium acetate as mobile phases.The 32 components were simultaneously analyzed in switching positive and negative modes.The retention time,accurate mass of parent ion and relative isotope abundance in full scan mode were used for fast screening of multi components in hair dyes,while accurate mass of fragment ions in two stage threshold triggered full mass scan mode were used for confirmation.Under the optimal conditions,good linearities(r2>0.99)were obtained in their respective concentration ranges.The limits of quantitation(LOQ)for 32 compounds were in the range of 0.2-40 μg/g.The recoveries ranged from 81.0% to 110% with relative standard deviations(RSDs)of 3.3%-9.9%.The method was simple,sensitive and accurate,and was suitable for the rapid screening and confirmation of 32 kinds of banned and restricted component in hair dyes.
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