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| Advances in Application of Polymer Coatings in Protein Separation by Capillary Electrophoresis |
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| DOI： |
| KeyWord:capillary electrophoresis polymer coatings protein separation electroosmotic flow |
| Author | Institution |
| CHEN Li-juan,YAN Ye-han,ZHU Hai-kun,MUHAMMAD Atif |
1.College of Materials and Chemical Engineering,West Anhui University;2.School of Chemistry and Materials Science,University of Sciences and Technology of China |
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| Abstract: |
| Capillary electrophoresis(CE),a highly mature and versatile technique,has been continually evolving in the last three decades.Capillary electrophoresis has important applications in the field of biological analysis such as protein separation due to its advantages of short separation time,high separation efficiency and low sample consumption,etc.However,many drawbacks have been caused by dissociation of silicon hydroxyl on inner wall of capillary in CE separation process.To overcome these drawbacks associated with CE,modification of capillary inner surfaces by antifouling polymer coatings are chosen.The antifouling polymer modified capillary method could not only regulate the electroosmotic flow,but also benefit the proteins separation.This method could also effectively improve the separation efficiency and separation stability for proteins in CE process.The paper presents a review on the latest research progress of capillary coatings,like dynamic and static polymer coating for protein separation.It also summarizes the research progress of polymer coating capillary based on dopamine/polydopamine anchored.And finally,the prospects of the future development trend of polymer coatings of capillary are discussed. |
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