Rapid Determination of Six Anesthetics Residues in Aquatic Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-through Solid Phase Extraction
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KeyWord:anesthetics  pass-through solid phase extraction  aquatic products  high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)
GAO Ping,YANG Xi,MO Cai-na,CHEN Ri-meng,ZENG Dan-dan,LIU Huan-ming,HONG Peng-zhi,ZHOU Kai,CHEN Ying-shou 1.Zhanjiang Institute for Food and Drug Control;2.Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture and Rural Affairs,Guangdong Evergreen Feed Industry Co.,Ltd.;3.Aquatic Product Processing and Safety Key Lab of Guangdong Province,College of Food Science and Technology,Guangdong Ocean University
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      A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS) method was developed for the rapid determination of six anesthetics,eg.procaine,lidocaine,bupivacaine,tetracaine,tricaine methanesulfonate(MS-222) and benzocaine in aquatic products.The samples were extracted with water and acetonitrile containing 1.0% formic acid,and then cleaned up with a PRiME HLB pass through solid phase extraction column.The targets were separated on a ZORBAX Eclipse XDB-C18 column(3.0 mm×150 mm,3.5 μm) with acetonitrile-0.1% formic acid as mobile phase by gradient elution.The analytes were determined in electrospray positive ionization(ESI+) mode under multiple reaction monitoring(MRM).Results showed that there were good linear relationships for the targets in their respective concentration ranges with correlation coefficients(r2) of 0.993 8-0.998 7.The limits of detection(LOD) and limits of quantitation(LOQ) were in the ranges of 1.5-6.0 μg/kg and 5.0-20 μg/kg,respectively.Average spiked recoveries for six anesthetics were in the range of 76.8%-110% with relative standard deviations of 4.2%-11%.The proposed method is simple,quick,accurate and sensitive,and is suitable for the determination of six anesthetics in aquatic products.