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| Non-directional Screening of Veterinary Drug Residues in Deep processed Lamb Products Based on Mass Spectrometry Fragmention |
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| DOI： |
| KeyWord:ultra-high performance liquid chromatography-hybrid quadrupole-orbitrap mass spectrometry(UHPLC-Q-Orbitrap MS) non-directional screening veterinary drug residues deep processed lamb products |
| Author | Institution |
| JIA Wei,XU Xi,SHI Lin,HE Bo,FAN Cheng,CHU Xiao-gang |
1.School of Food and Biological Engineering,Shaanxi University of Science and Technology;2.Key Laboratory of Se enriched Products Development and Quality Control,Ministry of Agriculture and Rural Affairs;3.Shaanxi Testing Institute of Product Quality Supervision;4.Chinese Academy of Inspection and Quarantine |
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| Abstract: |
| An analytical method was developed for the non directional screening of 34 residues in 7 categories of veterinary drugs by ultra high performance liquid chromatography-hybrid quadrupole-orbitrap mass spectrometry(UHPLC-Q-Orbitrap MS) based on mass spectrometry fragmentation.The qualitative screening of the analytes was performed by MS in a data independent acquisition mode,based on retention time,accurate mass number,secondary fragmentation information of 34 veterinary drug standard substances.The characteristic fracture fragments of 7 categories of veterinary drugs were obtained by exploring the molecular ion mass spectrometry rupture process and cracking mechanism.A method for untargeted screening of target and non target substances in deep processed lamb products was established by combining the compound database with characteristic ion fragments in the data independent collection mode.Results showed that there were good linear relationships for 34 veterinary drugs in respective concentration ranges with their correlation coefficients(r2) larger than 0.99.The limits of detection and quantitation were 0.04-4.76 μg/kg and 0.07-8.24 μg/kg,respectively.Average recoveries at three added levels ranged from 79.4% to 110%,with relative standard deviations(RSD) of 0.30%-7.5%.The method was applied in the non directional screening of veterinary drug residues in commercially available deep processed lamb samples,and difloxacin outside the standard library was also detected beside sulfamethazine.Hence,the proposed method is simple and efficient,and could be employed for accurate determination of veterinary drug residues in lamb products. |
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