Preparation of Ti4+ Immobilized Silica Nanocomposites and Their Application in Separation and Enrichment of Phosphoproteins
  
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KeyWord:“thiol-ene” click reaction  “one pot” process  phosphoproteins  nano sized SiO2  separation and enrichment
  
AuthorInstitution
XIE Qing,HU Qiao-yun,ZHENG Qiong,LIN Zi-an 1.Department of Pharmacy,Fujian Vocational College of Bioengineering;2.Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology,College of Chemistry,Fuzhou University
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Abstract:
      The Ti4+ immobilized silica nanocomposites(SiO2-Ti4+) were fabricated by synthesizing silica nanoparticles with tetraethoxysilane(TEOS) and γ-mercaptopropyl trimethoxysilane(γ-MPTS) as precursors,followed by “thiol-ene” click reaction and “one pot” process.The morphology and structure properties of the resultant SiO2-Ti4+ were characterized by transmission electronic microscopy(TEM),Fourier transform infrared spectroscopy(FT-IR) and X ray photoelectron spectroscopy(XPS).The adsorption behavior of the SiO2-Ti4+ towards phosphoproteins was investigated.Under the optimized conditions,the SiO2-Ti4+ showed a rather higher adsorption capacity towards phosphoproteins(α-casein,12.27 μmol/g) than non phosphoproteins(horseradishperoxidase,1.12 μmol/g).In addition,the practicality of the SiO2-Ti4+ was further demonstrated by their selective enrichment of phosphoproteins from milk samples.
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