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| Key Parameters for Molecular Weight Determination and Glycoform Identification on Intact Therapeutic Monoclonal Antibodies by Ultra high Resolution Orbitrap Mass Spectrometry with Nanospray Ionization |
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| DOI： |
| KeyWord:monoclonal antibody native mass spectrometry intact protein post translational modification glycoform |
| Author | Institution |
| DAI Rong-rong，MA Xin，SHAO Xin-yang，CHAIHU Ling-xiao，WANG Guan-bo |
1.School of Chemistry and Materials Science，Nanjing Normal University，Nanjing，China;2.Novo Nordisk Research Centre China，Beijing，China;3.Academy for Advanced Interdisciplinary Studies，Peking University，Beijing，China;4.Institute for Cell Analysis，Shenzhen Bay Laboratory，Shenzhen ，China |
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| Abstract: |
| Molecular weight determination and glycoform identification are the essential characterization data in stages ranged from development to quality control of therapeutic monoclonal antibodies(mAbs)，while determinations on mAbs at the intact protein level have been demonstrated to provide straightforward and valid data，which require instruments with high resolving power and involve sophisticated parameter settings.To reduce possible artifacts，impacts of parameters were investigated，including nominal resolution，in source fragmentation，collision induced dissociation and solution composition on the molecular weight determination and glycoform identification of intact Abs by orbitrap mass spectrometry with nanospray ionization in native mass spectrometric mode，so as to provide a useful reference for this kind of mass spectrometric analysis. |
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