|
| Determination of Mycophenolic Acid in Human Plasma by High Performance Liquid Chromatography with Online Solid Phase Extraction Purification |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:mycophenolic acid human plasma online solid phase extraction-high performance liquid chromatography(online SPE-HPLC) |
| Author | Institution |
| XU Ze-jun，JING Xia，SUN Fang，JI Chao，YANG Xin-lei |
1.Childrens Hospital of Nanjing Medical University，Nanjing，China;2.Agilent TechnologiesChina Co.，Ltd，Shanghai，China |
|
| Hits: 2085 |
| Download times: 1282 |
| Abstract: |
| An online solid phase extraction-high performance liquid chromatographic(Online SPE-HPLC) method was developed for the determination of mycophenolic acid in human plasma.Plasma samples were directly injected into the online SPE-HPLC system after simple protein precipitation.Capcell PAK MF Ph-1 was employed as an online SPE column and Poroshell 120 EC-C18 was employed as an analysis column.Results showed that there was a good linear relationship for mycophenolic acid in the concentration range of 020-5000 μg/mL,with a correlation coefficient(r) of 0999 8.The limit of detection(LOD) and limit of quantitation(LOQ) were of 007 μg/mL and 020 μg/mL,respectively.The spiked recoveries for mycophenolic acid in human plasma at three spiked levels of 039,2500 and 5000 μg/mL ranged from 962% to 105%,with intra assay relative standard deviations(intra RSD) of 26%-31% and inter assay relative standard deriation(inter RSD) of 29%-33%.The method was rapid and simple,and was suitable for the determination of mycophenolic acid concentrations in human plasma. |
| Close |