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| Detection of Copper Ions Based on Copper/Silver Nanoclusters |
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| DOI： |
| KeyWord:fluorescent sensing metal nanoclusters fluorescence enhancement copper ion DNA |
| Author | Institution |
| SUN Ya-lan，CAI Yi-na，REN Bing-xue，PENG Chi-fang |
1.School of Food Science and Technology,Jiangnan University;2.Food Inspection and Quarantine Technnology Centre,Shenzhen Customs |
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| Hits: 2010 |
| Download times: 941 |
| Abstract: |
| A fluorescent method based on copper/silver nanoclusters for the detection of Cu2+ was established by designing DNA sequences with G rich bases and investigating the fluorescence enhancement effect of G base pairs on DNA-Cu/AgNCs.It was found that the fluorescence intensity of the prepared DNA-Cu/AgNCs was significantly enhanced by the G5 sequence at the 5′ end of the C rich sequence template.Meanwhile,the fluorescence of DNA-Cu/AgNCs was quenched by Hg2+ and Cu2+.NaBH4 could be used to mask Hg2+ in order to achieve a selective detection of Cu2+.The linear range of this method for Cu2+ was from 0.01 μmol/L to 5.0 μmol/L,and the detection limit was 5.0 nmol/L.The method is simple,rapid,highly selective and low cost,and is able to apply in detection for real samples. |
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