Determination of Selenium Speciations in Human Urine by High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry
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KeyWord:human urine  selenium speciation  high performance liquid chromatography-inductively coupled plasma mass spectrometry
LIU Yuan,CHEN Shao-zhan,CHEN Zhen,YAO Xiao-hui,ZHOU Tian-hui,LIU Li-ping 1.School of Public Health,Capital Medical University;2.Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing Center for Disease Prevention and Control;3.Center for Preventive Medicine Research
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      A high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP/MS) was developed for the determination of five speciations of selenium,i.e.selenocystine(SeCys2),methylselenocysteine(MeSeCys),selenite[Se(Ⅳ)],selenomethionine(SeMet) and selenate[Se(Ⅵ)] in human urine.The samples were diluted with ultra pure water,and separated on a Hamilton PRP-X100 column(250 mm×4 mm,10 μm) by isocratic elution using 40 mmol/L diammonium hydrogen phosphate containing 1% methanol(pH 5) as mobile phase.Five selenium speciations could be separated within 13 min.There were linear relationships for five selenium speciations in the range of 0-300.0 μg/L with correlation coefficients(r) larger than 0.999.The detection limits of the method were 0.2-0.5 μg/L.Recoveries for MeSeCys,Se(Ⅳ),SeMet and Se(Ⅵ) were in the range of 80.0%-123%,except that the recovery for SeCys2 was 37.7%-70.4%.The relative standard deviations(RSD) were not more than 7.8%.Results showed that SeCys2 was the main selenium speciation in human urine,and a small amount of MeSeCys,SeMet,inorganic selenium and some unknown selenium compounds were also found.