Determination of Three Volatile Organic Compounds in Pond Water and Vegetables by High Performance Liquid Chromatography with Fluorescence Derivatization and Magnetic Dispersive Solid Phase Extraction
  
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KeyWord:volatile organic compounds  fluorescence derivatization  magnetic dispersive solid phase extraction  high performance liquid chromatography  environmental water samples  vegetables
  
AuthorInstitution
CHEN Kun,ZHANG Peng,WANG Yan,ZHENG Zhen-jia,DONG Jing-lei,SUN Lu-ping,ZHU Shu-yun,ZHAO Xian-en 1.Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine,College of Chemistry and Chemical Engineering,Qufu Normal University;2.College of Food Science and Engineering,Shandong Agricultural University;3.Longda Foodstuff Group Co.,Ltd
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Abstract:
      A new method of high performance liquid chromatography-fluorescence detection(HPLC-FLD) was developed for the detection of 2-methylisoborneol(2-MIB),geosmin(GSM) and 3-methyl-1-butanol(3-MB),with 6-carbonyl chloride levofloxacin(LFC-Cl) as derivatization reagent and magnetic graphene oxide(MGO) as magnetic dispersive solid phase extraction(MDSPE) adsorbent.Through optimizing derivatization conditions,three kinds of volatile organic compounds(VOCs) could be completely derivatized in 0.05 mol/L 4 dimethylaminopyridine(DMAP)acetonitrile solution within 90 min at 60 ℃ under ultrasonic assisted conditions.Through optimizing MDSPE conditions,three VOCs derivatives could be enriched and purified by a 20 min extraction of 20 mg adsorbent followed by a 3 min desorption of acetonitrile containing 1% formic acid.Under the optimized chromatographic conditions,the baseline separation and high sensitive detection of three VOCs derivatives were realized within 15 min.The limits of detection(LOD) and limits of quantitation(LOQ) of this method were in the ranges of 0.020-0.95 ng/L and 0.10-3.3 ng/L,respectively.The linearity,precision and recoveries were satisfactory.Compared with the reported methods,this method had the advantages of high sensitivity,simple sample pretreatment and universal instruments.It was suitable for the rapid determination of three VOCs in pond water and vegetables,providing a new approach for the monitoring of food and environmental water samples.
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