|
| Construction of a Two dimensional Photonic Crystal Hydrogel Sensor with Glycoproteins Imprinting |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:concanavalin A(Con A) photonic crystals molecular imprinting hydrogels debyering sensor |
| Author | Institution |
| LI Yan-xia,HUANG Lu,CHEN Yi-ting,LOU Ben-yong,MIAO Ling |
1.Department of Chemical Engineering,Ocean College,Minjiang University;2.Research and Testing Center of Pharmaceutical for Mulation,Minjiang University |
|
| Hits: 1790 |
| Download times: 893 |
| Abstract: |
| A two dimensional photonic crystal molecularly imprinted hydrogel(MIPs) sensor with specific recognition to glycoproteins was prepared by using concanavalin A(Con A) as the imprinted molecule,methylidenebisacrylamide(MBA) as the cross linking agents,acrylamide(AAm) and 4 vinylbenzeneboronic acid as the functional monomers,tetramethylethylenediamine(TEMED) as an initiator and ammonium persulfate(APS) as a catalyst.Polymerization was carried out to form a Con A imprinted hydrogel on the surface of two dimensional photonic crystal.After elution with 10% acetic acid solution,a MIPs sensor for specific recognition to Con A was constructed.Results showed that the diameter of Debye ring changed most significantly,and the target object was the most sensitive to be identified when the cross linking degree of MIPs was 10%.The sensor could be used in the enrichment and real time detection of target molecules as it had a good response to Con A in the concentration range of 0-05 mg/mL.Moreover,the introduction of phenyl boronic acid increased the recognition sites of the imprinted cavities,and further improved the specific recognition of the MIPs toward the carbohydrate molecules.The hydrogel sensor presented a good selectivity when binding to the proteins in ovalbumin(OVA),bovine hemoglobin(BHb),lysozyme(Lyz),trypsin(Try) solution under the same concentration.This method is easy to operate,low cost,reusable and needless for any special equipments.Furthermore,it could be extended to the real time and fast detection for other glycoproteins. |
| Close |
|
|
|