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| Evaluation of Fusion Fingerprint for Tuire Jiedu Injection by Comprehensive Linear Quantitative Fingerprint Method |
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| DOI： |
| KeyWord:comprehensive linear quantitative fingerprinting method Tuire Jiedu injection multi component quantification multi wavelength fusion method |
| Author | Institution |
| ZHANG Yue,LAN Li-li,GONG Dan-dan,SUN Guo-xiang |
School of Pharmacy,Shenyang Pharmaceutical University |
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| Abstract: |
| This study introduced the three parameters of comprehensive linear qualitative similarity Sl, comprehensive linear quantitative similarity Pl% and fingerprint variation coefficient α to establish a novel method comprehensive linear fingerprinting method(CLFM),which comprehensively evaluated the overall quality of traditional Chinese medicine from a qualitative and quantitative perspective.The fingerprints for Tuire Jiedu injection(TJI) samples at 210,254,265,330 and 360 nm wavelengths were collected by high performance liquid chromatography with diode array detection(HPLC/DAD),and followed by the data fusion.Meanwhile,four index components,including chlorogenic acid(CHA),forsythin A(FA),paeonol(PA) and saikosaponin D(SD) were quantitatively analyzed.The quality of the samples was identified by CLFM.Results showed that 10 batches of injection samples were divided into 5 levels,and 10 batches of intermediate samples were divided into 4 levels.The quantitative analysis results of the four index components were highly correlated with the quantitative similarity Pl% of the fingerprint,indicating that the CLFM could be used to replace the standard substance for sample quantitative analysis.This method provides a new idea for the overall quality control of traditional Chinese medicine. |
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