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| Sensitive Detection of Aflatoxin B1 in Traditional Chinese Medicine Using an Aptasensor Based on Enzyme-assisted Target Recycling |
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| DOI： |
| KeyWord:aflatoxin B1(AFB1) chemiluminescence target recycling G quadruplex DNAzyme traditional Chinese medicine |
| Author | Institution |
| WU Hao，WU Jun，WANG Hong-yong，LIU Ya-ling，HAN Guo-qing，LI Wen-chao，SHI Qing-yun，ZOU Pei |
1.NHC Key Laboratory of Nuclear Medicine，Jiangsu Key Laboratory of Molecular Nuclear Medicine，Jiangsu Institute of Nuclear Medicine;2.School of Pharmacy，Nanjing Medical University |
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| Abstract: |
| In this paper，a chemiluminescence aptasensor for the highly sensitive detection of aflatoxin B1 (AFB1) was constructed based on the strategy of enzyme assisted target recycling amplification.The label free aptamer probe H1-S1 and hairpin probe H2 were well designed using G-quadruplex/hemin DNAzyme as the signal molecule.The aptamer probe bound to the target AFB1，which triggered the target recycling amplification reaction with the help of exonuclease I to generate hairpin H1.The hairpin H1 was hybridized with H2，releasing the complete G-quadruplex sequence.The G quadruplex sequence combined with hemin to form G quadruplex/hemin DNAzyme，which catalyzed the oxidation of luminol-H2O2 chemiluminescence system to generate chemiluminescence signals to achieve the amplified detection of AFB1.Under the optimal experimental conditions，the chemiluminescence intensity exhibited a good linear relationship(r2=0.995 5) with the logarithm of AFB1 concentration in the range of 0.001-100 ng/mL.The detection limit was 0.93 pg/mL，and the recoveries were in the range of 93.7%-107%.With the advantages of simple operation，high sensitivity and good specificity，this aptasensor has a good application prospect in the detection of aflatoxin contamination. |
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