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| Scientific PapersA Novel Nanoliposome Signal Amplification Platform for Sensitive Personal Glucose Meter Based Point-of-Care Detection of Ricin |
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| DOI： |
| KeyWord:point of care testing nanoliposome ricin B-chain(RTB) personal glucose meter(PGM) |
| Author | Institution |
| MEN Chen,LI Yuan-fang,HUANG Cheng-zhi,ZHEN Shu-jun |
1.Key Laboratory of Luminescence Analysis and Molecular SensingSouthwest University,Ministry of Education,College of Chemistry and Chemical Engineering,Southwest University,Chongqing,China;2.Key Laboratory of Luminescent and Real Time Analytical ChemistrySouthwest University,Chongqing Science and Technology Bureau,College of Pharmaceutical Sciences,Southwest University,Chongqing,China |
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| Abstract: |
| Ricin is one of the most deadly toxins known,which could be used as a biological weapon causing potential environmental and food pollution.However,common methods for ricin detection are time consuming and limited due to their complicate and professional laboratory operations.To overcome these limitations of the common detection methods and achieve a simple,rapid and sensitive non laboratory detection of ricin,herein,a novel method was presented for the point of care(POC) detection of ricin B chain(RTB) by using amyloglucosidase loaded nanoliposome(ALN) to amplify the personal glucose meter(PGM) signal.Initially,a hybrid probe was constructed by hybridizing an aptamer on magnetic beads(MBs) with a blocker on ALN,combining the functions of target recognition,magnetic separation and signal amplification together.Then,in the presence of RTB,the blocker linked ALN was released from the surface of MBs after magnetic separation with the formation of RTB aptamer complex.Subsequently,as obtained ALN supernatant was lysed with Triton X-100 to liberate amyloglucosidase to hydrolyze amylose,generating a great number of glucose.Finally,the glucose was quantitatively read out by the PGM in 10 s.Therefore,RTB can be detected by using the enhancement of PGM value.The linear range for the detection was from 0.3 μg/mL to 30 μg/mL and the limit of detection(LOD) was 67 ng/mL.Results showed that the developed method remarkably improved the sensitivity and selectivity for RTB detection,and exhibited a great potential in POC detection of other targets. |
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