|
| Research Progress in Detection Methods for African Swine Fever |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:African swine fever detection methods virus separation methods immunological detection methods molecular detection methods |
| Author | Institution |
| YANG Zhan-sen，JIANG Ya-nan，CHENG Nan，LI Xiang-yang，HUANG Kun-lun，LIU Qing-liang，SUN Yan-li，XU Wen-tao |
1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing,China;2.College of Food Science and Engineering,Beijing University of Agricultural,Beijing,China;3.Shandong Baier Testing Co.,Ltd.,Weifang,China |
|
| Hits: 1543 |
| Download times: 546 |
| Abstract: |
| African swine fever is a devastating and highly contagious disease affecting pigs of all ages and breeds,which is characterized by high fever,cyanosis of the skin and severe hemorrhages in lymph nodes,with a mortality up to 100%.The World Organization for Animal Health(International Epizootic Office,OIE) has classified it into a kind of notifiable animal disease,while China has listed it as a predominantly exotic animal disease.since the African swine fever was reported to be endemic in sub-Saharan African countries in the 1920s,it has gradually become prevalent in the Europe,South America and Caucasus region of Russia,and broke out all over the world.It has caused a huge threat to swine industry and resulted in large economic losses.African swine fever virus,the causative agent of the African swine fever,is a double-stranded DNA arbovirus which is the only member of the family Asfarviridae,genus Asfivirus with icosahedral morphology and an average diameter of 200 nm.At present,there is no specific medicine or vaccine available to prevent African swine fever virus,and the main control strategy mainly relies on implement of sanitary measures and slaughtering of infected and exposed animals.Therefore,early diagnosis and detection on African swine fever virus is vitally important for epidemic identification and control.In this article,the detection methods for African swine fever virus are divided into virus separation methods,immunological detection methods and molecular detection methods according to the detection principles.The immunological detection methods are subdivided into immunofluorescence test,immunoblotting test,enzyme linked immunosorbent assay,immunochromatographic test strip and other immunological detection methods.The molecular detection methods are subdivided into polymerase chain reaction,isothermal amplification techniques,CRISPR(clustered regularly interspaced short palindromic repeats)/Cas system and other molecular detection methods.The principles and characteristics of these methods are introduced in this article,and the research progress in detection of African swine fever virus is summarized.The future development direction of detection methods for African swine fever virus is determined as there still remain some deficiencies of these methods in detection ability and application scenarios,of which the detection limits and the application ability for Point-of-Care diagnosis will improved in the future,based on the precondition ensuring sensitivity and specificity.The future research will focus on lowering the cost,industrializing production and improving the throughput and performance. |
| Close |
|
|
|