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| Quantitative Analysis of 12 Kinds of Long chain Triacylglycerols in Vegetable Oils by Ultra Performance Convergence Chromatography-Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:ultra performance convergence chromatography-tandem mass spectrometry long-chain triacylglycerols vegetable oils |
| Author | Institution |
| YANG Guang-yong，GUO Cang-ting，XUE Guang，GUO Jin-xi |
1.Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute,Urumqi,China;2.Urumqi Center for Disease Control and Prevention,Urumqi,China |
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| Abstract: |
| Long-chain triacylglycerols(TAGs) play an important role in maintaining human health and normal development,so it is necessary to establish a rapid and effective method for accurate determination of the contents of TAGs in edible vegetable oils.The polarities and structures of TAGs are very similar,which makes it difficult to separate them by either gas chromatography or liquid chromatography.A method based on ultra performance convergence chromatography-tandem mass spectrometry(UPC2-MS/MS) was established for the quantitative analysis of 12 kinds of TAGs in vegetable oils.The samples were firstly dissolved and diluted with n-hexane,then separated by gradient elution using an analytical column combining two HSS C18 SB(100 mm×2.1 mm,1.8 μm) columns together,with supercritical carbon dioxide and methanol containing 0.1% formic acid as mobile phases at a flow rate of 1.5 mL/min.The separated compounds were analyzed by MS/MS with an electrospray ionization source in positive ions and multiple reaction monitoring(MRM) modes.Water containing 97% methanol and 0.2% ammonia water was used as the auxiliary pump mobile phase at a flow rate of 0.3 mL/min.The external standard method was used for quantitative analysis of the 12 TAGs in sample solutions.Results showed that there were good linear relationships for the 12 TAGs in the corresponding mass concentration ranges with their correlation coefficients(r2) not less than 0.990 2.The limits of detection(LOD,S/N=3) and limits of quantitation(LOQ,S/N=10) were in the ranges of 1.0-8.0 mg/g and 3.0-25 mg/g,respectively.The average recoveries(n=9) of the method ranged from 95.7% to 104%,with relative standard deviations of 1.5%-4.2%,and good precisions.The developed method was successfully used for the determination of 12 long-chain TAGs in actual samples.Furthermore,some long-chain TAGs outside the scope of the investigation were analyzed.The method is high specific,rapid,simple,reliable,environmentally friendly and compatible for oils matrixes,providing an effective way for determining the contents of TAGs in vegetable oils,and it is also suitable for the analysis of TAGs in other samples. |
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