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| Construction of a New Fluorescent Probe Based on 1，4-Nucleophilic Addition Reaction for Sulfite and Its Application in Food and Living Cells |
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| DOI： |
| KeyWord:sulfite fluorescent probe food cell imaging |
| Author | Institution |
| ZHANG Chun-xiang，TANG Si-ping，LI Wen-qian，SHEN You-ming，GU Biao |
1. College of Chemistry and Materials Engineering， Hunan University of Arts and Science， Changde ， China;2. College of Chemistry and Materials Science， Hengyang Normal University; Key Laboratory of Functional Metal－Organic Compounds of Hunan Province， Hengyang ， China |
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| Abstract: |
| In this study， a simple but effective fluorescent probe（CQ） was constructed for the detection of sulfite. The N-methylquinoline trifluoromethanesulfonate fragment as the sulfite response site was linked to the 4-cyanobenzoacetonitrile fragment using a C? C bond. Upon treatment with sulfite， the probe CQ showed an obvious fluorescence enhancement. Meanwhile， the color of the CQ solution changed from colorless to yellow， which could be directly observed with naked eye. The reaction mechanism of probe CQ towards sulfite was studied in detail by 1H NMR spectra. Notably， the fluorescence response of the probe to sulfite was very fast（20 s）， which was suitable for the real-time detection of sulfite. In PBS buffer solution（DMF∶H2O=1∶9， volume ratio， pH 7.4）， there existed a good linear relationship between the fluorescence intensity at 511 nm and concentration of sulfite in the range of 0－10 μmol/L， with a detection limit of 25 nmol/L. The selectivity experiments indicated that probe CQ has a high selectivity for sulfite， which could satisfy the requirements for practical application. More importantly， the probe could not only be applied to the detection of sulfite in real food samples， but also realize the imaging of sulfite living cells， which provides a powerful analytical method for the monitoring of sulfite in food and biological systems. |
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