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| Quantitative Determination of Lactulose in Liquid Milk by High Performance Liquid Chromatography with Evaporative Light Scattering Detection |
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| DOI： |
| KeyWord:lactulose high performance liquid chromatography evaporative light scattering liquid milk |
| Author | Institution |
| SUN Pei， REN Shuo， CHEN Rou-han， GU Shuqing，NIU Bing，DENG Xiao-jun |
1. Department of Food Engineering， School of Life Science， Shanghai University， Shanghai ， China;2. Technical Center for Animal Plant and Food Inspection and Quarantine of Shanghai Customs， Shanghai ， China;3. Shanghai Center of Agri-products Quality and Safety， Shanghai ， China |
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| Abstract: |
| Lactose and lactulose in liquid milk are isomers with similar retention properties， which makes separation and detection on lactulose by chromatography difficult. At present， although a variety of liquid chromatography detection methods have been reported， there are some problems in the simplicity of the pretreatment， the applicability of the chromatographic column and the stability of the separation effect. A method for the determination of lactulose in liquid milk by high performance liquid chromatography with evaporative light scattering detection（HPLC－ELSD）was developed. The protein was deposited with 0.4 mol/L ammonium acetate（pH 3.8）. The sugar analysis column was used for separation， and the evaporative light scattering detector was applied to detection. There was a good linear relationship for lactulose in liquid milk in the range of 1.0－100 mg/L， with a correlation coefficient（r2） of 0.998 0. The detection limit and the quantitative limit were 15 mg/L and 50 mg/L， respectively. The recoveries were in the range of 85.1%－110%， with relative standard deviations（RSDs） not more than 7.5%. The method is simple to operate， and could be completed within 8 min. It could meet the requirements for daily detection of lactulose with good repeatability and high accuracy. |
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