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| Detection of 17β-Estradiol in Water by Timeresolved Fluorescence Lateral Flow Assay |
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| DOI： |
| KeyWord:time-resolved fluorescence lateral flow assay（LFA） 17β-estradiol fluorescence resonance energy transfer persistent luminescence particle |
| Author | Institution |
| WANG Ling-ling，WANG Wen-long，YANG Cheng， XU Zheng-hua，ZHANG Yi，SHI Xue-li |
1. State Key Laboratory of Food Science and Technology，Jiangnan University，Wuxi ，China;2.Institute of Analytical Food Safety，School of Food Science and Technology，Jiangnan University，Wuxi ， China; 3. Huangpu Customs of the People's Republic of China，Guangzhou ，China; 4. Shijiazhuang Maternal and Child Health Hospital，Shijiazhuang ，China |
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| Abstract: |
| A competitive time-resolved fluorescence（TRF） lateral flow assay for 17β-estradiol（E2） was established with 17β-estradiol－BSA（E2－BSA） coupled persistent luminescence particles（PLPs） as fluorescence donor and colloidal gold（CG）-labelled antibody as fluorescence receptor，based on immune recognition and fluorescence resonance energy transfer principle．In this method，the PLPs with long persistent luminescence but large size of micron were applied as the fluorescence signal source，and its non-migration property on nitrocellulose membrane was used to fix the E2 competitor in the test zone，thus the contradiction between the limitation that the signal material of traditional test paper must be nanometer size and the short luminous lifetime of small PLPs was skillfully solved．In addition，a low-power UV lamp was used as the light source to excite the PLPs，and the continuous shooting function of smart phone was used to realize TRF－mode fluorescence acquisition，effectively removing the fluorescent background interference from the test strips and improving the signal-to-noise ratio．The detection limit of this method was 0.1 ng/mL，which was about two orders of magnitude more sensitive than those of colloidal gold and HPLC－FLD method．The detection limit of this method was 0.1 ng/mL，which was about two orders of magnitude more sensitive than that of colloidal gold．The method showed good selectivity and could perform detection in 30 min．Using this method to detect E2 in a water sample and the results were consistent with that of HPLC-FLD method. |
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