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| Determination of Triazine and Phenylurea Herbicides in Soy Milk by High Performance Liquid Chromatography with Hydrophobic Deep Eutectic Solvent Based Liquid－Liquid Microextraction |
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| DOI： |
| KeyWord:liquid－liquid microextraction deep eutectic solvents triazine herbicides phenylurea herbicides soy milk high performance liquid chromatography |
| Author | Institution |
| ZHU Qing，LONG Bing-hua，CHEN Xing-hang，YAN Xu，ZHU Hong-nian，FENG Ji，XU Wei-li，WANG Zhi-bing |
College of Chemistry and Life Science，Changchun University of Technology，Changchun ，China |
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| Abstract: |
| A novel high performance liquid chromatography（HPLC） with hydrophobic deep eutectic solvent（DES） based liquid－liquid microextraction was developed for the separation and determination of triazine（atrazine，terbutryn） and phenylurea（monuron，chlortoluron） herbicides in market packaged soymilk．In this work，a series of new DESs with different molar ratios were synthesized with tetrabutylammonium chloride as hydrogen bond acceptor and hexafluoropropanol as hydrogen bond donor．In addition，the experimental conditions that affect the extraction effect were investigated in detail，including type and amount of DESs，amount of NaCl，vortex time， pH value and temperature．Experimental results showed that，there were good linear relationships for the target analytes in the range of 1.00－500.00 μg/L，with correlation coefficients（r） not less than 0.998 4．The limits of detection（LOD，S/N = 3） and quantitation（LOQ，S/N = 10） were in the range of 0.56－0.95 μg/L and 1.87－3.16 μg/L，respectively．The relative standard deviations for intra-day and inter-day precision were in the range of 0.28%－2.0% and 2.1%－7.5%，respectively．Recoveries for the target analytes in four spiked samples ranged from 86.4% to 117%，with relative standard deviations of 0.13%－5.7%.With the advantages of simplicity，rapidness，short extraction time，low consumption and low experimental cost，this method could be used for the analysis and detection of triazine and phenylurea herbicides in market packaged soy milk． |
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