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| Analytical Methods for Localization of Methylated DNA Modification |
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| DOI： |
| KeyWord:methylated DNA modification 5?methylcytosine location analysis sequencing |
| Author | Institution |
| CHEN Meng?yuan,YOU Xue?jiao,YUAN Bi?feng,FENG Yu?qi |
1. College of Chemistry and Molecular Sciences，Wuhan University，Wuhan ，China;
2. School of Public Health，Wuhan University，Wuhan ，China |
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| Abstract: |
| As the most widely studied epigenetic modification in DNA，5?methylcytosine（5mC） plays an important role in the growth and development of organisms．5mC participates the regulation of gene expressions without changing the sequences of genes．Investigation on the localization and content changes of 5mC in the incidence of diseases will strengthen our understanding toward its roles in the development of diseases．Elucidation of the biological functions of 5mC mainly depends on uncovering its accurate localization information in genomes．Over the past few decades，many analytical methods based on high?throughput sequencing technology have been established to localize 5mC in genomes．Apart from the classic BS?seq（bisulfite sequencing）?，some other analytical methods，such as DIP?seq（DNA immunoprecipitation sequencing）?，EM?seq（enzymatic methyl?seq）??，TAPS（TET?assisted pyridine borane sequencing）?，nanopore?seq（nanopore sequencing） and SMRT?seq（single molecule real?time） have been developed．In this review，the principles，advantages and disadvantages of these analytical methods for 5mC localization in DNA based on the high?throughput sequencing technology are summarized and discussed.In addition，the future research directions for mapping 5mC in DNA are also envisioned.It is hoped that this review will benefit and stimulate the study of the biological functions of 5mC in genomes. |
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